Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

879
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
879
Labeling DNA Probes03:31

Labeling DNA Probes

9.0K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Classification of "Athlete's Heart" using machine learning of conventional 12-lead ECG: male elite 3,000-m runner data in the CHIEF study.

Frontiers in sports and active living·2026
Same author

Comparison of the outcomes of targeted temperature management for the cardiac arrest patients with and without diabetes mellitus: The TIMECARD study.

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same author

Impact of Fluticasone Furoate/Vilanterol on Small Airway Function in Asthma: A Real- World Study Using Impulse Oscillometry Stratified by Eosinophilic Inflammation.

Journal of asthma and allergy·2026
Same author

Migration-Enhanced Synthesis of 2D Copper Telluride Ultrathin Thermoelectrics.

ACS nano·2026
Same author

Endotypic traits and lung function in patients with obstructive sleep apnea comorbid with obesity hypoventilation syndrome.

Sleep & breathing = Schlaf & Atmung·2026
Same author

Comparative efficacy of mepolizumab and benralizumab in severe eosinophilic asthma: a retrospective cohort study.

BMC pulmonary medicine·2026
Same journal

Ti/Sr Gradient Doping with SrTiO<sub>3</sub> Coating for Mitigating Strain and Oxygen Loss in Ni-Rich Cathode.

ACS applied materials & interfaces·2026
Same journal

Metallic Lead to Perfect Perovskite: A Bottom-Up Vapor-Assisted Colloidal Strategy for High-Performance Solar Cells.

ACS applied materials & interfaces·2026
Same journal

Two-Dimensional VSe<sub>2</sub>@Polypyrrole Heterostructure Enables Stable High-Rate Lithium-Sulfur Batteries.

ACS applied materials & interfaces·2026
Same journal

A Multifunctional Hydrogel Integrating Hemostatic, Antioxidant, and Antibacterial Properties for Infected and Diabetic Wound Regeneration.

ACS applied materials & interfaces·2026
Same journal

Tunable Interfacial to Filamentary Resistive Switching Mechanism in Room-Temperature-Grown Amorphous YBa<sub>2</sub>Cu<sub>3</sub>O<sub><i>x</i></sub> with Excess Cu Addition.

ACS applied materials & interfaces·2026
Same journal

Bioinspired Rhombic VO<sub>2</sub> Metasurface with Low Solar Absorptance for Self-adaptive All-Weather Building Thermal Management.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Dec 5, 2025

Low-energy Cathodoluminescence for OxyNitride Phosphors
07:03

Low-energy Cathodoluminescence for OxyNitride Phosphors

Published on: November 15, 2016

11.0K

Multilevel Optical Labeling by Spectral Luminescence Control in Nanodiamond Color Centers.

Yan-Ting Chuang1, Sheng-Ding Chen2, Wei-Chun Huang3

  • 1Graduate Institute of Applied Physics, Nation Taiwan University, 106 Taipei, Taiwan.

ACS Applied Materials & Interfaces
|October 16, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel multicolor bioimaging technique using fluorescent nanodiamonds. This method distinguishes labels by emission intensity, overcoming spectral overlap limitations for enhanced imaging resolution.

Keywords:
NV centerbioimagingbiolabelingmemorynanodiamond

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.9K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

454

Related Experiment Videos

Last Updated: Dec 5, 2025

Low-energy Cathodoluminescence for OxyNitride Phosphors
07:03

Low-energy Cathodoluminescence for OxyNitride Phosphors

Published on: November 15, 2016

11.0K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.9K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

454

Area of Science:

  • Nanotechnology
  • Bioimaging
  • Optical Physics

Background:

  • Current multicolor bioimaging is limited by fluorophore spectral overlap, hindering resolution.
  • Distinguishing multiple optical labels is essential for advanced bioimaging applications.

Purpose of the Study:

  • To develop a novel method for distinguishing optical labels by emission intensity, overcoming spectral overlap.
  • To demonstrate a robust and reproducible multicolor imaging approach using fluorescent nanodiamonds.

Main Methods:

  • Utilized highly functionalized fluorescent nanodiamonds with controllable emission properties.
  • Implemented an optical writing process involving selective photobleaching of N3 color centers via a two-photon-assisted dissociation process.
  • Developed a spectral feature for normalization to account for sample variability.

Main Results:

  • Achieved multilevel optical labels by permanently modifying spectral response through laser irradiation.
  • Demonstrated selective photobleaching of N3 centers while maintaining stable nitrogen vacancy (NV) emission.
  • Created "write once read many" (WORM) memory with robust and reproducible multilevel storage.
  • Successfully characterized markers in HeLa cells with high fidelity amidst complex emission backgrounds.

Conclusions:

  • The proposed method enables high-fidelity multicolor bioimaging by distinguishing labels via emission intensity.
  • This approach overcomes limitations of spectral overlap and offers robust, reproducible multilevel optical labeling.
  • Demonstrated direct manipulation of label information within cells, paving the way for advanced bioimaging techniques.