Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry
Alexander Leitner1, Alexandre M J J Bonvin2, Christoph H Borchers3
1Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.
Structure (London, England : 1993)
|October 16, 2020
Summary
Cross-linking mass spectrometry (MS) is a vital tool for understanding protein structure and interactions. This paper calls for developing standardized methods and reporting guidelines for cross-linking MS to ensure consistent and reliable results.
Area of Science:
- Biochemistry
- Proteomics
- Structural Biology
Background:
- Cross-linking mass spectrometry (MS) has significantly advanced over 20 years.
- It is widely used for protein structure determination, conformation analysis, and interaction mapping in complex biological samples.
- The method is a routine and appreciated tool, particularly in structural biology.
Framework:
- This white paper arises from an open community process initiated in 2015.
- It identifies key areas within cross-linking MS that require standardized guidelines.
- The goal is to establish a formal Cross-linking MS standards initiative.
Implementation:
- The paper consolidates community input from various conference events.
- It focuses on aspects of cross-linking MS methodology needing standardization.
- The initiative aims to foster consistent practices across research labs.
Implications:
- Developing methodological and reporting standards is timely for the cross-linking MS community.
- Standardization will enhance the reliability and reproducibility of cross-linking MS experiments.
- This initiative will support the continued growth and application of cross-linking MS in scientific research.
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