Related Experiment Video
Updated: Dec 5, 2025

Protocol to Create Chronic Wounds in Diabetic Mice
Published on: September 25, 2019
Failure of Healing in Chronic Diabetic Wounds: A Case Report
1James B. Stiehl, MD, MBA, is an orthopedic surgeon, St Mary's Hospital, Centralia, Illinois. Acknowledgments: The author thanks Morgan Mulvany, RN, Administrative Director of Odin Care, Odin, Illinois; and Erick Rivas, MD, Vohra Physicians, Chicago, Illinois. The author has disclosed no financial relationships related to this article. Submitted April 1, 2020; accepted in revised form May 13, 2020.
Abstract:
Stalled healing in chronic wounds is a challenging problem for providers and remains multifactorial in etiology. Older adults with insulin-dependent diabetes are at very high risk. In this case report, two patients with large nonhealing wounds were considered for treatment with daily jet lavage irrigation in an attempt to remove the inflammatory products of their respective chronic wounds and eliminate the persisting biofilm bacteria. Several attempts were made to reduce treatments to two to three times per week, and negative-pressure wound therapy was initiated in both cases only to see the return of inflammation and necrosis of the wound bed. In both cases, the daily jet lavage irrigation was successful in creating a granulating wound bed that slowly healed over many months. One patient died with an open sacral pressure injury, and the other patient died 4 months after complete healing of a large heel pressure injury. The interesting observation is the necessity of daily high-intensity wound irrigation to correct the chronic infectious process. Diabetic chronic wounds in high-risk older adults are recalcitrant to standard wound treatments, and providers should consider daily jet lavage wound irrigation to deal with this problem.

