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Updated: Dec 5, 2025

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Published on: April 2, 2012
Corps nucléaires PML, centromères et contrôle de la latence du virus herpès simplex 1
1Virus & Centromère, Centre de génétique et physiologie moléculaire et cellulaire, CNRS, UMR5534, Université Claude-Bernard Lyon 1, 16, rue Raphaël-Dubois, Bâtiment Gregor-Mendel, 69100 Villeurbanne, Université de Lyon 1, 69000 Lyon, France, Laboratoire d'excellence, LabEX DEVweCAN, 69000 Lyon, France.
Abstract:
After a primary infection, many viruses establish a latent infection and stay invisible for the host immune system until reactivation. To understand how a virus seemingly « under control » could reactivate and induce pathology, it is essential to understand the different cellular mechanisms implicated in the antiviral defense. Promyelocytic leukemia (PML) nuclear bodies (PML-NB) are nuclear relays of the antiviral response implicated in the nucleus-associated intrinsic antiviral defense. Many viruses interfere with the activity of the PML-NB, however not much is known about the capacity of these domains to interact with the nucleus incoming viral genomes. This review describes how a recent study of my team has enabled to decipher, in a physiological context, the role of the PML-NB in the detection, structuration and transcriptional control of the herpes simplex virus 1 (HSV-1). It opens new perspectives to understand how the antiviral response associated with nuclear domains could control many other viruses.
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