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Updated: Dec 5, 2025

Colony Forming Cell CFC Assay for Human Hematopoietic Cells
Published on: December 18, 2010
[Biological Characteristics of Immunophenotype, Stem Cell Gene and Colony Forming Ability in Side-Population Cells of
Shi-Xia Zhou1, Li Zhang2, Xiao-Ming Li3
1Department of Hematology, Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province, China,Stem Cell Laboratory Affiliated Hospital of Southwest Medical Univeristy, Luzhou 646000,Sichuan Province, China.
Abstract:
AbstractObjective: To investigate the in vitro biological characteristics of side-population(SP) cells in mantle cell lymphoma(MCL).
Methods:
The SP cells in JeKo-1 cells were enriched by continuous culture and sorted by flow cytometry(FCM), and the expression of CD19 and IgM were analyzed. The differences of gene expression and their self-renewal ability between SP cells and non-SP cells were estimated by qRT-PCR and colony-forming assay(CFA) respectively.
Results:
SP cells in JeKo-1 cells could be enriched by continuous sorting and in vitro cultureing, and it was found that CD19-/IgM- accounted for (2.79±0.82)%,CD19-/IgM+accounted for (70.99±13.61)%,CD19+/IgM-accounted for (0.55±0.25)%, and CD19+/IgM+accounted for (25.67±14)%. Bmi-1,CD133, C-MYC and Nanog genes were highly expressed in SP cells, but were low expressed in non-SP cells (P<0.05). Compared with non-SP cells, SP cells had stronger colony forming ability (P<0.05).
Conclusion:
JeKo-1 cell line of mantle cell lymphoma contains SP cells, which can be enriched by continuous sorting and culture for research. The SP cells in JeKo-1 contain four different cell populations with different immunophenotypes: CD19-/IgM-、CD19-/IgM+、CD19+/IgM-、CD19+/IgM+, and show stronger gene expression of stem cells and self-renewal ability in vitro.
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