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Updated: Nov 10, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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[Effect of Autophagy to Drug Resistance of B-cell Lymphoma Cells]

Shi-Xia Zhou1, Li Zhang2, Xiao-Ming Li3

  • 1Department of Hematology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China,Stem Cell Laboratory Affiliated to Southwest Medical University, Luzhou 646000, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|April 4, 2021
PubMed
Abstract

Insights

Autophagy enhances drug resistance in lymphoma cells. Inhibiting autophagy, a cellular process, significantly reduces resistance to chemotherapy drugs like ADR and VCR in these cells.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Medicine

Background:

  • Drug resistance remains a significant challenge in lymphoma treatment.
  • Autophagy, a cellular degradation process, has been implicated in various diseases, including cancer.
  • Understanding the role of autophagy in lymphoma drug resistance is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the role of autophagy in the drug resistance of human lymphoma cell lines.
  • To determine the correlation between autophagy activity and resistance to Adriamycin (ADR) and Vincristine (VCR) in lymphoma cells.

Main Methods:

  • Utilized three human lymphoma cell lines: Burkitt's lymphoma (Daudi), B lymphoma (SUDHL-4), and mantle cell lymphoma (JeKo-1).
  • Manipulated autophagy activity by using autophagy inhibitors and stable Atg5 interference via lentivirus infection.
  • Assessed changes in autophagy activity and resistance to ADR and VCR chemotherapy drugs.

Main Results:

  • Lymphoma cell resistance to ADR and VCR correlated positively with their basal autophagy activity, with JeKo-1 cells exhibiting the highest resistance and autophagy.
  • Inhibition of autophagy through chemical inhibitors or genetic Atg5 interference significantly diminished the drug resistance of all tested lymphoma cell lines.
  • A direct positive correlation was observed between the level of autophagy activity and the degree of drug resistance in B lymphoma cells.

Conclusions:

  • Autophagy plays a significant role in mediating chemotherapy drug resistance in human lymphoma cells.
  • Inhibiting autophagy presents a potential therapeutic approach to overcome drug resistance in lymphoma treatment.
  • Targeting autophagy could enhance the efficacy of conventional chemotherapy agents like ADR and VCR in lymphoma patients.

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