Sludge ratio affects the start-up performance and functional bacteria distribution of a hybrid CANON system

To investigate the effect of sludge ratio on the hybrid CANON system, autotrophic nitrogen removal sludge was inoculated with different granule/floc ratios to initiate the CANON system, and maintained the sludge ratio during the operation process. The start-up performances were compared, and the distribution characteristics of functional bacteria were investigated. The results show that the Equivalent system (granules:flocs = 1:1-1:1.5) successfully started-up on day 19, and the nitrogen removal rate (NRR) reached 0.299 kgN m^-3·d^-1 on day 63. At the same time, it was less affected by the load shock than High-granules and High-flocs systems. Therefore, the Equivalent system had the strongest start-up performance. The activities of the functional bacteria conformed to spatial heterogeneity, unlike the abundance. With the increased floc proportion, the difference in the activity and abundance of anaerobic ammonium-oxidizing bacteria (AAOB) between the granules and flocs increased, while there was a decrease in the difference in aerobic ammonium-oxidizing bacteria (AOB). However, the abundance of Nitrosomonas in the granules was higher than in the flocs when the proportion of flocs was higher than 50%. This study provides new ideas and insights for the fast start-up of the CANON system and can conform to the varying needs of engineering applications.