Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Method for histological preparation of bone sections containing titanium implants.

J A Hipp, J B Brunski, G V Cochran

    Stain Technology
    |July 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Epithelial Rests of Malassez Form a Wnt-Dependent Mechanoresponsive Network.

    Journal of dental research·2026
    Same author

    Wnt/β-catenin Signaling Controls Maxillofacial Hyperostosis.

    Journal of dental research·2022
    Same author

    Characterization of the mid-coronal plane method for measurement of radiographic change in knee joint space width across different levels of image parallax.

    Osteoarthritis and cartilage·2021
    Same author

    Interspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone.

    JDR clinical and translational research·2020
    Same author

    Mechanoadaptive Responses in the Periodontium Are Coordinated by Wnt.

    Journal of dental research·2019
    Same author

    Biomechanics of Immediate Postextraction Implant Osseointegration.

    Journal of dental research·2018

    A new hand-grinding technique creates thin bone-titanium implant sections for detailed analysis. This method minimizes sample damage and is cost-effective for researchers studying bone integration.

    Area of Science:

    • Biomaterials Science
    • Histotechnology
    • Orthopedic Research

    Background:

    • Analyzing bone-titanium implant interfaces is crucial for understanding osseointegration.
    • Existing sectioning methods can cause sample damage, hindering accurate analysis.
    • Novel techniques are needed to preserve the integrity of delicate bone-implant tissues.

    Purpose of the Study:

    • To develop a cost-effective thin sectioning technique for bone-titanium implant sites.
    • To enable clear visualization of bone labels and the tissue-implant interface.
    • To minimize sample cracking and distortion during the sectioning process.

    Main Methods:

    • Hand grinding was employed to produce 20-40-micron-thick bone sections.
    • Surface staining with oxytetracycline-HCl and DCAF allowed visualization under transmitted light.

    Related Experiment Videos

  • A pneumatic press and methyl methacrylate monomer ensured bubble-free, strong bonding to acrylic slides.

    • Main Results:

    • The technique successfully produced thin sections of bone-titanium implant sites.
    • Bone labels and the tissue-implant interface remained intact and visible.
    • Minimized sample cracking and distortion were observed.
    • The bonding method enhanced section integrity and grinding properties.

    Conclusions:

    • This hand-grinding technique offers a viable, low-cost method for preparing bone-titanium implant sections.
    • The technique preserves critical histological details for research and analysis.
    • It provides an accessible option for laboratories needing to study bone-implant interfaces.