Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A solid-phase antiglobulin test.

R Douglas, J V Schneider, D Wilkie

    Transfusion
    |September 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    The solid-phase test (SPT) offers comparable sensitivity to the indirect anti-human globulin test (IAHG) for detecting IgG antibodies. This machine-readable adaptation shows good correlation with manual compatibility testing methods.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Safety and Efficacy of Atezolizumab in Combination With nab-Paclitaxel in Patients With PD-L1 Positive Metastatic or Locally Advanced Triple-Negative Breast Cancer: A UK-Wide Cancer Centre Experience.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2025
    Same author

    Combining QSAR and SSD to predict aquatic toxicity and species sensitivity of pyrethroid and organophosphate pesticides.

    SAR and QSAR in environmental research·2024
    Same author

    Position paper on olfactory dysfunction: 2023

    Rhinology·2023
    Same author

    Towards an action plan for characterizing food plant diversity.

    Nature plants·2022
    Same author

    A historical review of the evolution of nasal lavage systems.

    The Journal of laryngology and otology·2021
    Same author

    Characterization of transient noise in Advanced LIGO relevant to gravitational wave signal GW150914.

    Classical and quantum gravity·2020

    Area of Science:

    • Immunology
    • Transfusion Medicine
    • Clinical Pathology

    Background:

    • The indirect anti-human globulin test (IAHG) is a standard method for detecting antibodies in blood compatibility testing.
    • Manual IAHG testing can be time-consuming and subject to inter-observer variability.
    • There is a need for automated, machine-readable methods to improve efficiency and consistency in antibody detection.

    Purpose of the Study:

    • To describe and evaluate a machine-readable solid-phase test (SPT) as an adaptation of the manual IAHG test.
    • To compare the sensitivity and accuracy of the SPT with the manual IAHG test for detecting IgG antibodies.
    • To assess the correlation between SPT results and manual pretransfusion compatibility tests.

    Main Methods:

    • Development of a solid-phase test (SPT) as a machine-readable adaptation of the indirect anti-human globulin test (IAHG).

    Related Experiment Videos

  • Comparison of SPT sensitivity with manual IAHG testing for IgG antibody detection.
  • Evaluation of SPT performance using blood samples from donors and hospitalized patients, with comparison to manual compatibility tests.
  • Analysis of the relationship between SPT optical density values and manual IAHG scores.

    • Main Results:

    • The SPT demonstrated comparable sensitivity to manual IAHG testing for IgG antibody detection.
    • A linear and overlapping relationship was observed between manual IAHG scores and SPT optical density values.
    • Compatibility testing on over 1700 samples showed good correlation between SPT and manual IAHG results, with minor discrepancies (1.38% SPT+/IAHG- and 5.37% SPT-/IAHG+).
    • Optimal immunoglobulin concentration for the solid phase was approximately 275 mg/L, with accuracy slightly decreasing at higher red cell concentrations.

    Conclusions:

    • The solid-phase test (SPT) is a viable, machine-readable alternative to the manual indirect anti-human globulin test (IAHG).
    • The SPT offers comparable sensitivity and good correlation with established manual compatibility testing methods.
    • This automated approach has the potential to enhance efficiency and consistency in pretransfusion compatibility testing.