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Related Experiment Video

Updated: Dec 5, 2025

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

High DNA integrity sperm selection using surface acoustic waves.

Junyang Gai¹, Reza Nosrati¹, Adrian Neild¹

¹Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia. Reza.Nosrati@monash.edu Adrian.Neild@monash.edu.

|October 19, 2020

Summary

This summary is machine-generated.

This study introduces an automated acoustic-based method for selecting high-quality sperm, significantly improving motility and DNA integrity for assisted reproductive technologies like in vitro fertilization (IVF). The new technique offers a faster, more efficient alternative to traditional sperm selection processes.

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Area of Science:

Reproductive Biology
Biotechnology
Bioengineering

Background:

Male infertility affects a significant portion of the global population.
Current sperm selection methods are labor-intensive and time-consuming, limiting their clinical effectiveness.
There is a need for efficient and automated sperm selection techniques for assisted reproductive technologies.

Purpose of the Study:

To develop and validate an automated, continuous-flow acoustic-based method for sperm selection.
To assess the improvement in sperm quality, including motility and DNA integrity, using the developed method.
To provide a clinically relevant number of high-quality sperm for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedures.

Main Methods:

An acoustic field was utilized in a continuous-flow microfluidic device to separate sperm.
Sperm were guided across the channel width based on their size and motility within the acoustic field.
The method was applied to raw bull semen for automated sperm selection.

Main Results:

The acoustic-based method successfully selected high-quality sperm with significantly improved motility and DNA integrity compared to raw semen.
Sperm vitality and progressive motility showed over 50% and 60% improvement, respectively.
DNA integrity improved by more than 38%, with over 60,000 sperm selected in under an hour.

Conclusions:

Automated acoustic-based sperm selection offers a rapid and efficient approach to improving sperm quality.
This method enhances sperm vitality, motility, and DNA integrity, making it suitable for IVF and ICSI.
The technology presents a promising advancement for male infertility treatment and reproductive medicine.