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Related Experiment Video

Updated: May 25, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

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Open Microfluidic Cell Culture in Hydrogels Enabled by 3D-Printed Molds.

Madison O'Brien1,2, Ashley N Spirrison1, Melati S Abdul Halim3

  • 1Jim and Joan Bock Department of Biomedical Engineering, Trine University, Angola, IN 46703, USA.

Bioengineering (Basel, Switzerland)
|February 26, 2025
PubMed
Summary

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Researchers developed 3D-printed molds for open microfluidic cell cultures, creating tissue mimics like blood vessels. This simpler method enhances accessibility for in vitro studies and disease mechanism investigations.

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Microfluidics

Background:

  • In vitro cell culture models are crucial for studying cellular behavior and disease mechanisms.
  • Existing microfluidic systems can be complex and require specialized equipment.
  • There is a need for accessible, versatile cell culture platforms that mimic physiological structures.

Purpose of the Study:

  • To develop a simplified method for creating open microfluidic cell cultures using 3D-printed molds.
  • To demonstrate the fabrication of various tissue-mimicking structures in different hydrogels.
  • To validate the use of these structures for in vitro biological studies, including modeling blood vessels.

Main Methods:

  • Utilized 3D-printed molds to create open microfluidic channels and wells in hydrogels (agarose, gelatin, collagen type I).
Keywords:
3D-printed moldsblood vessel mimicscollagenhydrogelopen microfluidics

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Last Updated: May 25, 2025

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  • Fabricated open channels with varying diameters (400 µm to 4 mm) and collagen densities (2-4 mg/mL).
  • Generated blood vessel mimics using human umbilical vein endothelial cells (HUVECs) in open channels and subjected them to hypoxia.

    • Main Results:

    • Demonstrated successful fabrication of diverse geometries, including open channels and various well shapes, in multiple hydrogels.
    • Achieved high cell viability (>89%) in blood vessel mimics with diameters of 800 µm and 2 mm.
    • Observed significant reductions in cell viability (8.3%) and CD31 expression (7.4%) under hypoxic conditions compared to normoxia.

    Conclusions:

    • The 3D-printed mold method offers a simpler, more accessible approach to creating versatile in vitro tissue mimics.
    • This technique enables the generation of physiologically relevant structures for studying cellular responses to various conditions, such as hypoxia.
    • The developed platform holds promise for advancing in vitro research in cell biology, disease modeling, and drug discovery.