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Related Experiment Videos

Cytomat-R: a computer-controlled multiple laser source multiparameter flow cytophotometer system.

H M Shapiro, E R Schildkraut, R Curbelo

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |July 1, 1977
    PubMed
    Summary
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    A new multiparameter flow cytophotometer system uses multiple laser wavelengths for advanced cell analysis. This system enables detailed cell characterization, counting, and classification for research applications.

    Area of Science:

    • Biomedical Engineering
    • Optical Physics
    • Cell Biology

    Background:

    • Flow cytometry is a crucial technique for cell analysis.
    • Existing flow cytometers have limitations in simultaneous multi-wavelength illumination and data acquisition.
    • Advancements are needed for more comprehensive cellular interrogation.

    Purpose of the Study:

    • To develop and present a novel multiparameter flow cytophotometer system.
    • To enable simultaneous multi-wavelength illumination for enhanced flow cytometry.
    • To facilitate the development of new flow cytometric techniques.

    Main Methods:

    • The system utilizes multiple laser sources for illumination.
    • It employs a general-purpose digital computer for control.

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  • It integrates multiple detectors for light scattering, extinction, and fluorescence measurements.

    • Main Results:

    • The system allows simultaneous illumination of different flow chamber regions with three laser wavelengths.
    • Up to five optical measurements (scattering, extinction, fluorescence) are acquired per illuminated station.
    • The system processes cells at 1000 cells/sec, recording seven correlated optical parameters per cell.

    Conclusions:

    • The developed multiparameter flow cytophotometer system offers advanced capabilities for cell analysis.
    • It supports the development and implementation of sophisticated cell characterization, counting, and classification procedures.
    • The system is suitable for diverse basic and clinical research applications.