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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Dec 5, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Continuous microfluidic 3D focusing enabling microflow cytometry for single-cell analysis.

Sheng Yan¹, Dan Yuan²

¹Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, China.

|October 20, 2020

Summary

This summary is machine-generated.

Microfluidic 3D focusing enhances microflow cytometry for detailed single-cell analysis. This advanced technique offers superior capabilities for understanding cellular heterogeneity in biology and medicine.

Keywords:

Flow cytometry Microfluidics Single-cell analysis Three-dimensional focusing

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Last Updated: Dec 5, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

Single-cell analysis aims to understand cellular heterogeneity.
Microflow cytometry offers high-throughput, biochemical single-cell analysis using microfluidic focusing.
Advancements in microfabrication enable 3D microfluidic focusing, improving flow cytometry sensing.

Purpose of the Study:

To review recent developments in microfluidic technologies for 3D cell focusing.
To discuss applications of 3D microfluidic focusing in advanced microflow cytometry.
To explore challenges and future perspectives of 3D microfluidic focusing in single-cell analysis.

Main Methods:

Review of microfluidic technologies for 3D cell focusing based on underlying physics.
Discussion of applications in impedance, optical, imaging, and deformability flow cytometry.
Analysis of challenges and future outlook for microfluidic 3D focusing.

Main Results:

Microfluidic 3D focusing significantly improves sensing capabilities in flow cytometry.
3D focusing enables new types of advanced microflow cytometry.
Applications span impedance, optical, imaging, and deformability flow cytometry.

Conclusions:

Microflow cytometry with 3D focusing demonstrates superior capabilities for single-cell analysis.
This technology is highly promising for future applications in biology and medicine.
Further research will expand its utility in diverse scientific fields.