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Multi-targeted loop mediated amplification PCR for diagnosis of extrapulmonary tuberculosis
Kusum Sharma1, Shreya Singh1, Megha Sharma1
1Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
The Indian Journal of Tuberculosis
|October 20, 2020
Summary
Multi-targeted loop mediated amplification (MLAMP) offers a rapid and robust method for diagnosing extrapulmonary tuberculosis (EPTB). This technique improves sensitivity by targeting multiple genes, making it ideal for resource-limited settings.
Area of Science:
- Microbiology
- Molecular Diagnostics
- Infectious Diseases
Background:
- Extrapulmonary tuberculosis (EPTB) diagnosis remains challenging due to low bacterial loads.
- Current diagnostic methods often lack the required sensitivity and speed for timely intervention.
Purpose of the Study:
- To evaluate the efficacy of multi-targeted loop mediated amplification (MLAMP) for rapid EPTB diagnosis.
- To compare MLAMP performance against conventional diagnostic techniques.
Main Methods:
- A total of 700 patients were enrolled, including confirmed EPTB, suspected EPTB, and non-tubercular controls.
- Samples were analyzed using Ziehl-Neelsen microscopy, Lowenstein Jensen culture, IS6110 PCR, and individual/multi-targeted LAMP assays (IS6110 and MPB64).
Main Results:
- MLAMP demonstrated high sensitivity (86.6%) and 100% specificity.
- Compared to microscopy (12%) and culture (25%), MLAMP significantly improved detection rates.
- Single-target LAMP assays showed sensitivities of 80% (IS6110) and 86.6% (MPB64).
Conclusions:
- MLAMP is a rapid, robust, and sensitive tool for EPTB diagnosis.
- Utilizing dual targets (IS6110 and MPB64) enhances diagnostic yield in extrapulmonary samples.
- MLAMP's ease of use, speed, and low cost make it suitable for resource-limited settings.
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