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Time-gated fluorescence signalling under dissipative conditions.

Maria A Cardona1, Rui Chen1, Subhabrata Maiti2

  • 1Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy. leonard.prins@unipd.it.

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Researchers developed a new method for time-gated fluorescence signaling using an ATP-fueled self-assembly process. This approach allows for controlled, temporal emergence of functions in complex chemical systems, with potential for reactivation.

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Area of Science:

  • * Chemistry
  • * Chemical Biology
  • * Materials Science

Background:

  • * Biological systems exhibit precise temporal control over functions.
  • * Dissipative systems offer unique opportunities for dynamic chemical processes.

Purpose of the Study:

  • * To demonstrate time-gated fluorescence signaling using an ATP-fueled self-assembly process.
  • * To explore the temporal emergence of functions in synthetic chemical systems.

Main Methods:

  • * Utilized an adenosine triphosphate (ATP)-fueled self-assembly process.
  • * Established a temporal ATP-concentration gradient to control system states.
  • * Employed a hydrophobic dye for fluorescence detection within assemblies.

Main Results:

  • * The system exhibited three distinct states, with fluorescence observed only in the intermediate state.
  • * A temporal gradient of ATP concentration was crucial for triggering the fluorescent signal.
  • * The system demonstrated the ability to be reactivated by introducing additional ATP.

Conclusions:

  • * A strategy for programming the temporal emergence of functions in complex chemical systems was developed.
  • * Time-gated fluorescence signaling can be achieved under dissipative conditions.
  • * The self-assembly process provides a platform for dynamic and controllable chemical functions.