Prussian Blue (bio)sensing device for distance-based measurements

In this research, microfluidic paper distance-based systems for the quantification of redox species are proposed. For the preparation of the sensing zone a Prussian Blue (PB) (convertible to Prussian White (PW)) layer was deposited in the channel manufactured by wax-printing technique. According to the chemical properties of PB/PW system, it is possible to develop optical sensors sensitive to both oxidizing and reducing agents. The created systems were evaluated for the determination of ascorbic acid and hydrogen peroxide, which were chosen and used as model analytes. The final versions of the proposed systems exhibited a linear response from 0.25 mmol L^-1 to 4.0 and 2.0 mmol L^-1 for ascorbic acid and H₂O_2, respectively. The analytical utility of the paper systems was confirmed by measuring the levels of ascorbic acid in dietary supplements. Results correlation obtained for the described systems and the reference method was over 0.98 (Pearson's R-coefficient). All measurements were characterized by satisfactory reproducibility and acceptable uncertainty (RSD (%) < 6%). Finally, it was demonstrated that the modification of the PW-strip systems with oxidoreductase led to an enzymatic assay for glucose up to 10 mmol L^-1 range. Practical utility of the developed bio-strips was confirmed by quantifying glucose in drinks and dietary supplement samples.