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Prussian Blue (bio)sensing device for distance-based measurements.

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Summary

This study introduces novel microfluidic paper devices for quantifying redox species, demonstrating accurate measurements of ascorbic acid and hydrogen peroxide. These paper-based sensors show practical utility in analyzing dietary supplements and drinks.

Keywords:
Distance-based measurementsGlucosePaper-based microfluidicsPrussian bluePrussian white

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Microfluidic paper-based analytical devices (µPADs) offer low-cost, portable solutions for chemical analysis.
  • Prussian Blue (PB) and Prussian White (PW) systems are electrochemically active materials suitable for optical sensing.
  • Quantification of redox species like ascorbic acid and hydrogen peroxide is crucial in various applications.

Purpose of the Study:

  • To develop and validate microfluidic paper distance-based systems for quantifying redox species.
  • To utilize the Prussian Blue/Prussian White system for optical sensing applications.
  • To demonstrate the analytical utility of these paper-based systems for real-world sample analysis.

Main Methods:

  • Fabrication of microfluidic channels on paper using wax-printing.
  • Deposition of Prussian Blue (PB) layers for sensing zones.
  • Optical detection of redox species based on PB/PW chemical properties.
  • Modification of PW-strip systems with oxidoreductase for enzymatic assays.

Main Results:

  • Linear response ranges established for ascorbic acid (0.25–4.0 mmol L⁻¹) and hydrogen peroxide (0.25–2.0 mmol L⁻¹).
  • High correlation (Pearson's R > 0.98) with reference methods for ascorbic acid in dietary supplements.
  • Successful quantification of glucose (up to 10 mmol L⁻¹) using enzymatic bio-strips in drinks and supplements.
  • Satisfactory reproducibility (RSD < 6%) and acceptable measurement uncertainty.

Conclusions:

  • Microfluidic paper distance-based systems utilizing PB/PW are effective for quantifying redox species.
  • The developed systems demonstrate practical analytical utility for ascorbic acid and glucose in complex samples.
  • Enzymatic modification enhances the capability of paper-based sensors for specific analyte detection.