A novel petasin-modified zinc oxide eugenol sealer

Area of Science:

• Dental Materials Science
• Endodontics
• Biomaterials

Background:

• Zinc oxide eugenol (ZOE) is a widely used root canal sealer.
• ZOE exhibits drawbacks including cytotoxicity, solubility, and periapical tissue irritation.

Purpose of the Study:

• To evaluate the effects of incorporating petasin extract into ZOE sealer.
• Investigate modifications in setting time, solubility, cytotoxicity, and anti-inflammatory action.

Main Methods:

• Setting time assessed using Vicat's apparatus.
• Solubility tested per ADA specification #8.
• Cytotoxicity and anti-inflammatory effects evaluated using MTT assay and protein denaturation assay on L929 cell lines.

Main Results:

• The 5:1 ratio of ZOE with petasin extract showed the shortest setting times.
• No significant differences in solubility were observed across groups.
• The petasin-modified ZOE exhibited significantly lower cytotoxicity and higher anti-inflammatory effects compared to the control.

Conclusions:

• The 5:1 ratio of ZOE and petasin extract offers reduced setting time and cytotoxicity.
• This combination demonstrates superior anti-inflammatory properties compared to unmodified ZOE.