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Assessing false paternity risk in simulated motherless cases from more than 20 000 real exclusion trios
Vitor R C Aguiar1, Amanda M de Castro2, Laélia M Pinto2
1Departamento de Ciências Biológicas, Núcleo de Genética Humana e Molecular, Universidade Federal do Espírito Santo, Vitória, Brazil.
Background:
When the mother's DNA profile is not available for paternity testing, there is a smaller probability that a locus will exclude an alleged father. This study aims to evaluate the risk of potential false paternity inclusions in motherless cases.
Study Design And Methods:
More than 20 000 duos were generated by removing the maternal genotypes from exclusion trios. After recalculating paternity in these duos, any found inclusions would be false.
Results:
The use of an appropriate number of loci, mutation model, and mutation rates to analyze motherless paternity cases was robust against false inclusions. A single potential false inclusion was observed in a case wherein kinship plays a role. This result highlights the importance of testing the mother when available and of obtaining information on family circumstances for the proper handling of cases involving related individuals.
Conclusion:
The guidelines we used here were sufficient to avoid false inclusions in a data set of more than 20 000 motherless cases.
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