An Improved Protocol for the Synthesis and Purification of Yariv Reagents

Yariv reagents are glycoconjugate tris-azo dyes widely used in plant biology. These reagents are synthesized by diazo coupling between phloroglucinol and a para-diazophenyl glycoside. Despite their synthetic accessibility, well-defined protocols for obtaining pure Yariv reagents, and their complete compound characterization data, have not been reported. We report here optimized protocols used to synthesize, purify, and characterize a panel of six Yariv reagents and suggest approaches that could be valuable for the purification and characterization of other glycoconjugates as well.