Indocyanine Green Assembled Nanobubbles with Enhanced Fluorescence and Photostability
Li Yang1, Bin Huang2, Feng Chen3
1State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
Abstract:
Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye for extensive biomedical application. However, its fluorescence intensity is limited by its poor aqueous stability and concentration-dependent aggregation. To overcome these limitations, ICG self-assembled nanobubbles (ICG-NBs) with an average size of 244.6 nm are fabricated. In the ICG-NB assembled structures, the ICG molecules are arrayed on the gas-liquid interface by the hydrophobic interaction with the gas core and hydrophilic heads with water. Results show that ICG-NBs exhibited good monodispersity and excellent fluorescence and size stability. Compared with ICG solution, the ICG-NBs indicate the enhanced quantum yield and fluorescence intensity. The surface-enhanced Raman scattering (SERS) spectra and fluorescence lifetime measurement demonstrate that the ICG molecule assembled NBs could result in the changes of molecular vibration and time-resolved intensity decays of ICG. Thus, the ICG-NBs could be more beneficial for optical imaging in clinical applications in the future.
