Chimerism of chrysanthemum stems changes at the nodes during vegetative growth

The shoot apical meristem (SAM) is typically divided into three cell layers: the outermost epidermal layer (L1), the subepidermal layer (L2) and the inner corpus region (L3). Structures within the cell layers are normally maintained throughout development; however, through vegetative propagation of a periclinal chimeric chrysanthemum expressing a fluorescent protein gene only in the L1 layer, we collected twelve independent shoots that had partially mosaic fluorescent inner cells (L2, L3) in addition to fluorescent epidermal cells (L1). Furthermore, the elongated tissues of nine shoots out of the twelve had no internal fluorescent cells, i.e., they had the original L1 chimerism. Observations of the fluorescence distribution suggested that the change in chimerism occurred at the nodes, indicating previously unnoticed cell layer dynamics occurring at the nodes.