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Updated: Dec 4, 2025

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Advances in Lentivirus Purification.

Ana Sofia Moreira^1,2, David Guia Cavaco^1,2, Tiago Q Faria^1,2

¹iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, Oeiras, Portugal.

Biotechnology Journal

|October 22, 2020

Summary

This summary is machine-generated.

Lentiviral vectors (LVs) are crucial for gene therapies, but production demands higher yields and purity. This review details downstream processes, highlighting innovations for cost-effective manufacturing and expanded clinical applications.

Keywords:

bioseparation chromatography downstream processing lentiviral vectors

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Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Area of Science:

Biotechnology
Gene Therapy
Process Engineering

Background:

Lentiviral vectors (LVs) are key tools for gene and cell therapies due to stable genomic integration.
Significant advancements in LV production and purification have occurred over decades.
Growing demand for high-quantity, high-purity LVs necessitates novel manufacturing strategies.

Purpose of the Study:

To review and analyze downstream process unit operations for lentiviral vector manufacturing.
To identify limitations, strengths, and potential outcomes of current and emerging LV production methods.
To explore strategies for cost-effective LV supply to meet market demands.

Main Methods:

Detailed review of lentiviral vector downstream processing unit operations.
Analysis of manufacturing trends, including shifts from adherent to suspension cell cultures.
Examination of purification strategies, focusing on chromatography and membrane technologies.

Main Results:

Current large-scale LV manufacturing predominantly uses adherent cell cultures, with a rapid industry shift towards suspension cultures.
Standard purification involves batch chromatography and membrane technology.
Novel solutions are emerging to enhance current production schemes.

Conclusions:

Continuous innovation in downstream processing is vital for meeting the increasing demands for lentiviral vectors.
Optimizing production and purification strategies will facilitate the expanded clinical use of LVs.
Developing cost-effective manufacturing processes is essential for broader accessibility of LV-based therapies.