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Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

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Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Two-dimensional separation techniques using supercritical fluid chromatography.

Alexander S Kaplitz1, Mahmoud Elhusseiny Mostafa2, Samantha A Calvez1

  • 1Department of Chemistry & Biochemistry, Rowan University, Glassboro, NJ, USA.

Journal of Separation Science
|October 22, 2020
PubMed
Summary
This summary is machine-generated.

Two-dimensional chromatography using supercritical fluid chromatography (SFC) enhances complex mixture analysis. This review highlights SFC

Keywords:
chiral separationsmodulationmultidimensional separationssupercritical fluid chromatography

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • High-resolution separation is crucial for analyzing complex mixtures.
  • Multidimensional chromatography is a key strategy to increase separation resolution.
  • Supercritical fluid chromatography (SFC) offers unique advantages for multidimensional separations.

Purpose of the Study:

  • To review two-dimensional chromatography methods utilizing SFC over the past decade.
  • To discuss the interfacing of SFC with other chromatographic techniques.
  • To highlight applications and future directions of SFC in multidimensional separations.

Main Methods:

  • Focus on two-dimensional chromatography (2D-LC, 2D-GC) employing SFC.
  • Description of valving schemes and modulation strategies for SFC interfacing.
  • Review of recent literature on SFC-based multidimensional separations.

Main Results:

  • SFC's high solvent compatibility, rapid duty cycles, and orthogonality are beneficial for 2D separations.
  • Successful applications in analyzing oils and chiral separations of pharmaceuticals demonstrated.
  • Interfacing strategies for SFC with LC and GC are detailed.

Conclusions:

  • SFC is a powerful tool for advanced multidimensional separations.
  • Further development in interfacing and applications is anticipated.
  • SFC-based multidimensional chromatography offers significant potential for complex mixture analysis.