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Related Experiment Video

Updated: Dec 4, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

An origami electrical biosensor for multiplexed analyte detection in body fluids.

Yu Shen¹, Sidharth Modha², Hideaki Tsutsui³

¹Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA, 92521, USA.

Biosensors & Bioelectronics

|October 22, 2020

Summary

This study presents an affordable, sensitive paper-based microfluidic platform for rapid, multiplexed biomarker detection in body fluids. The cost-effective device enables point-of-care diagnostics with high specificity and user-friendliness.

Keywords:

ASSURED criteria FET/Chemiresistive biosensors Inkjet printing Multiplexed detections Paper-based microfluidics Single-walled carbon nanotubes

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Last Updated: Dec 4, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

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Published on: June 1, 2012

Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Current diagnostic methods often require complex laboratory equipment and trained personnel.
There is a need for rapid, cost-effective, and sensitive point-of-care diagnostic tools for various body fluids.

Purpose of the Study:

To develop an affordable, highly sensitive, and specific paper-based microfluidic platform for multiplexed biomarker detection.
To demonstrate the platform's capability for analyzing small sample volumes (microliter-sized) from diverse body fluids.
To achieve ultra-low detectable concentrations for target biomarkers.

Main Methods:

Fabrication of a paper-based microfluidic sensor array using wax barriers and 'paper bridges'.
Deposition of pyrene carboxylic acid-modified single-walled carbon nanotubes (PCA/SWNTs) via inkjet printing.
Immobilization of antibodies onto SWNTs for field-effect transistor (FET)/chemiresistor (CR) biosensors.
Optimization of sensing conditions for paper-based CR biosensors targeting human serum albumin (HSA) and human immunoglobulin G (HIgG).
Integration of origami folding for simplified fabrication.

Main Results:

Achieved ultra-low detectable concentrations of HSA and HIgG at 1.5 pM.
Demonstrated high sensitivity and specificity for target biomarker proteins.
The platform successfully performed multiplexed detections using only a microliter of sample.
The fabricated biosensors exhibited excellent performance characteristics.

Conclusions:

The developed paper-based microfluidic platform is cost-effective, user-friendly, and enables rapid, multiplexed biomarker detection.
The platform shows significant potential for point-of-care diagnostics, aligning with ASSURED criteria.
This technology offers a versatile solution for practical diagnostics and quick testing of targets of interest.