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Updated: Dec 4, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes
J Armando Casas-Mollano1, Matthew H Zinselmeier2, Samuel E Erickson1
1Department of Biochemistry, Molecular Biology, and Biophysics, BioTechnology Institute, University of Minnesota, Twin-Cities, Saint Paul, Minnesota, USA; and Cell Biology, and Development, University of Minnesota, Twin-Cities, Saint Paul, Minnesota, USA.
Abstract:
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
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