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Single-parent expression drives dynamic gene expression complementation in maize hybrids.

Zhi Li1, Peng Zhou2, Rafael Della Coletta1

  • 1Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA.

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|October 24, 2020
PubMed
Summary
This summary is machine-generated.

Single-parent expression (SPE) in maize arises from differential gene expression or gene absence. Genetic distance between parents correlates with SPE, with cis-regulation being dominant for both types.

Keywords:
complementationgene expression regulationmaizepresence/absence variationsingle-parent expressiontransposable element

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Area of Science:

  • Plant genetics and genomics
  • Gene expression regulation
  • Maize (Zea mays) research

Background:

  • Single-parent expression (SPE) describes genes expressed from only one parent.
  • SPE can result from non-presence/absence (non-PAV) differential expression or physical gene absence (PAV).
  • Understanding SPE mechanisms is crucial for plant breeding and hybrid vigor.

Purpose of the Study:

  • To compare the characteristics of PAV-SPE and non-PAV-SPE genes in maize.
  • To investigate the genetic and regulatory factors influencing different SPE types.
  • To assess the role of SPE in gene expression complementation in maize hybrids.

Main Methods:

  • Analysis of transcriptome data from 401 diverse maize inbreds and hybrids across five tissues.
  • Correlation analysis between parental genetic distance and SPE gene frequency.
  • Regulatory analysis focusing on cis and trans effects, including transposable elements.

Main Results:

  • SPE is highly genotype and tissue-specific, with higher incidence correlated with greater parental genetic distance.
  • Both PAV-SPE and non-PAV-SPE genes are primarily regulated by cis effects, influenced by polymorphic transposable elements.
  • PAV-SPE genes show higher expression in hybrids; silent allele expression in non-PAV-SPE hybrids can lead to non-additive expression.

Conclusions:

  • PAV-SPE and non-PAV-SPE exhibit distinct genetic and regulatory properties in maize.
  • Transposable elements significantly contribute to cis-regulation in both SPE types.
  • SPE plays a role in gene expression complementation, influencing hybrid performance in maize.