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Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes
Weronika Górka-Kumik1, Paula Garbacz, Dorota Lachowicz
1Jagiellonian University, Faculty of Physics, Astronomy and Applied Computer Science, Łojasiewicza 11, 30-348 Krakow, Poland.
Journal of Materials Chemistry. B
|October 25, 2020
Summary
Researchers developed novel magnetic scaffolds using poly(APTAC) brushes and superparamagnetic iron oxide nanoparticles (SPIONs) for non-invasive cell detachment and transfer, avoiding temperature-induced cell property changes.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- Stimuli-responsive polymers are used for cell culture scaffold detachment.
- Thermo-responsive polymers can alter cultured cell properties due to temperature changes.
Purpose of the Study:
- To present a novel, non-invasive cell detachment method using magnetic fields.
- To develop stimuli-responsive scaffolds based on poly(APTAC) brushes with embedded SPIONs.
Main Methods:
- Fabrication of hybrid scaffolds via surface-initiated atom transfer radical polymerization (SI-ATRP).
- Characterization using magnetic atomic force microscopy (AFM), secondary ion mass spectrometry (SIMS), and X-ray photoelectron spectroscopy (XPS).
- Culturing and characterization of neuroblastoma cells before and after magnetic field exposure.
Main Results:
- Successful creation of poly(APTAC) brushes with embedded SPIONs.
- Demonstration of magnetic field-triggered neuroblastoma cell detachment.
- Confirmation of non-invasive cell transfer without chemical or mechanical intervention.
Conclusions:
- Magnetic field-responsive scaffolds offer a non-invasive alternative for cell detachment and transfer in cell culture.
- This approach avoids the detrimental effects of temperature stimuli on cell properties.
