Related Experiment Video
Updated: Dec 4, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Chromatographic and spectroscopic determination of solvent-extracted Lantana camara leaf oil
1Faculty of Chemical and Food Engineering, Bahir Dar Institute of Technology, Bahir Dar University, Ethiopia.
Objective:
This study aimed to determine the chemical profile of Lantana camara leaf oil.
Methods:
The essential oil was extracted from dried leaf samples using the Soxhlet extraction method. The oil was separated from the solvent and the bioactive compounds were identified using gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared spectroscopy (FTIR). The identified peaks in the mass spectrum were matched with the database of the US National Institute of Standards and Technology (NIST) library.
Results:
The FT-IR results indicated the presence of alcohols, carboxylic acids, phenols, alkanes, ketones, and primary amine compounds. GC-MS identified 43 compounds representing 95% of the total leaf essential oil components. Some of the major isolated compounds included a pyrrolizine; 1-dodecanol; 1,2-nonadecanediol; phytol; 1,3-dioxolane; 4-undecene, 9-methyl, (Z)-; 1-eicosanol; and imidazole.
Conclusions:
The identified constituents of the extracted oil have established pharmacologic and insecticidal activities, and these compounds are also used in the drink, food, and cosmetic industries. This extract is highly valuable for the medical treatment of various ailments.
More Related Videos
07:29HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
09:48Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018