In Vitro Biostimulation of Low-Power Diode Pumping Solid State Laser Irradiation on Human Serum Proteins

This research is conducted to clarify whether the action of low-power diode pumping solid state (DPSS) laser doses modify proteins of normal human blood serum in vitro. Low-power laser light is considered to act through biostimulation rather than through thermal effects. It was found that low-power laser light biostimulates various biological processes, such as increasing the blood flow within the microcirculation. Human blood serum samples were carefully collected and divided into five equal aliquots. One of them served as a control (nonirradiated serum) and the other four aliquots were irradiated by DPSS laser at a wavelength of 589 nm with different doses (50, 70, 90, and 110 J/cm²). The electrophoretic migration speeds of each specific protein were measured immediately after irradiation using protein electrophoresis. A paired Student's t-test was used between variables. The protein concentrations were not significantly (p > 0.05) changed by the various doses of DPSS laser comparing with the nonirradiated counterpart. The electrophoretic migration speed of serum proteins was significantly decreased in almost all tested doses relative to the nonirradiated counterpart. Moreover, the irradiation of serum proteins (albumin, alpha1, alpha 2, beta, and globulin) with a laser dose of 70 J/cm² was associated with a significant decrease (p < 0.003, 0.02, 0.002, 0.02 and 0.001, respectively) in protein migration speed compared with the protein migration speed of the control nonirradiated counterpart. Laser light at a wavelength of 589 nm induces processes that lead to decreases in serum protein migration speeds. Globulin protein was found to have the lowest migration speed among the other plasma proteins.