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Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
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Lipopolysaccharide induces BV2 microglial cell migration via a decrease in SET8 expression.

Yanjun Zhao1,2, Wenjing Yi2, Yi Lu2

  • 1Putuo District People's Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

Canadian Journal of Physiology and Pharmacology
|October 27, 2020
PubMed
Summary
This summary is machine-generated.

SET8 regulates microglial migration by controlling Src expression, impacting inflammation. This study reveals SET8

Keywords:
BV2 cellsETS proto-oncogene 1ETS1SET8cell migrationcellules BV2lipopolysaccharidelipopolysaccharidesmigration cellulaire

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Area of Science:

  • Neuroinflammation research
  • Cellular signaling pathways
  • Molecular mechanisms of cell migration

Background:

  • Activated microglia contribute to tissue damage and chronic inflammation.
  • Src kinase is crucial for cell motility, particularly after lipopolysaccharide (LPS) exposure.
  • SET8 is implicated in various cellular signaling pathways.

Purpose of the Study:

  • To investigate the role of SET8 in lipopolysaccharide (LPS)-induced microglial migration.
  • To determine if SET8 regulates microglial migration via modulation of Src expression.
  • To elucidate the molecular mechanisms linking SET8, ETS1, and Src in microglial migration.

Main Methods:

  • Utilized BV2 microglial cell line.
  • Investigated the effects of LPS treatment on cell migration, Src expression, SET8, and ETS1 levels.
  • Employed overexpression and knockdown (shSET8) techniques for SET8 and ETS1.
  • Performed chromatin immunoprecipitation (ChIP) assays to analyze promoter enrichment.
  • Assessed Src promoter activity.

Main Results:

  • LPS promoted BV2 cell migration by increasing Src expression.
  • LPS decreased SET8 expression and increased ETS proto-oncogene 1 (ETS1) expression.
  • Overexpression of SET8 and knockdown of ETS1 reversed LPS-induced Src upregulation and migration.
  • SET8 was found to associate with ETS1.
  • SET8, ETS1, and H4K20me1 were enriched at the Src promoter region, with SET8 influencing Src promoter activity.

Conclusions:

  • SET8, in association with ETS1, regulates Src expression.
  • This SET8-ETS1-Src pathway is involved in lipopolysaccharide (LPS)-induced microglial migration.
  • Findings provide insights into the molecular mechanisms underlying neuroinflammation and microglial activation.