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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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LINC00210 exerts oncogenic roles in glioma by sponging miR-328

Zhifei Wang1, Hao Wu1, Hui Yan1

  • 1Department of Neurosurgery, Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.

Experimental and Therapeutic Medicine
|October 28, 2020
PubMed

Insights

Long non-coding RNA LINC00210 is upregulated in glioma, promoting cancer progression by sponging microRNA-328. This finding offers potential therapeutic targets for glioma treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in human cancers, including glioma.
  • The specific role of lncRNA LINC00210 in glioma remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and regulatory function of lncRNA LINC00210 in glioma cells.
  • To elucidate the underlying molecular mechanism of LINC00210 in glioma progression.

Main Methods:

  • Quantitative real-time PCR to assess LINC00210 expression in glioma tissues and cell lines.
  • Cell proliferation and migration assays (e.g., U251, T98G cells) following LINC00210 knockdown.
  • Luciferase reporter assays to confirm direct targeting of microRNA (miR)-328 by LINC00210.
  • MiR-328 mimic transfection to assess its role in modulating LINC00210 knockdown effects.

Main Results:

  • LINC00210 expression was significantly upregulated in glioma tissues compared to normal tissues.
  • High LINC00210 expression correlated with advanced clinical stage and poor prognosis in glioma patients.
  • LINC00210 knockdown inhibited glioma cell proliferation and migration.
  • LINC00210 directly targets miR-328, with inverse correlation in glioma tissues.
  • LINC00210 knockdown increased miR-328 expression, and miR-328 inhibition reversed the anti-tumor effects of LINC00210 knockdown.

Conclusions:

  • LINC00210 acts as an oncogenic lncRNA in glioma.
  • LINC00210 promotes glioma progression by sponging miR-328.
  • LINC00210-miR-328 axis represents a potential therapeutic target for glioma.
Keywords:
LINC00210gliomamicroRNA-328migrationproliferation

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