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Updated: Dec 3, 2025

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
Published on: March 3, 2016
Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
Li-Ping Liu1,2,3, Ning-Ning Guo1,2, Yu-Mei Li1,2
1Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China.
Abstract:
Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).
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