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Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Generation of Engineered Human Myocardium in a Multi-well Format
Malte Tiburcy1,2, Tim Meyer1,2, Norman Y Liaw1,2
1Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany.
STAR Protocols
|October 28, 2020
Summary
This study presents a method for generating engineered human myocardium (EHM) from pluripotent stem cells (PSCs). This scalable protocol enables efficient cardiac muscle production for drug screening and disease modeling applications.
Area of Science:
- Cardiovascular Biology
- Stem Cell Research
- Biotechnology
Background:
- Engineered human myocardium (EHM) is crucial for cardiac research.
- Existing methods for EHM generation can be limited in scalability and standardization.
Purpose of the Study:
- To describe a robust protocol for generating EHM from pluripotent stem cells (PSCs).
- To enable parallel culture of multiple EHM samples for high-throughput applications.
Main Methods:
- Utilized pluripotent stem cells (PSCs) for EHM generation.
- Employed defined, serum-free culture conditions in a multi-well plate format.
- Enabled parallel culture of up to 48 EHM samples.
Main Results:
- Successfully generated contractile engineered human myocardium.
- Demonstrated protocol applicability to human embryonic stem (HES) cells and induced PSCs.
- Showed efficacy with subtype-specific (atrial, ventricular) and commercial cardiomyocytes.
Conclusions:
- The protocol provides a scalable and defined method for EHM production.
- Generated EHM is suitable for diverse applications, including drug screening and disease modeling.
- This method supports advancements in cardiac regenerative medicine and pharmacology.
