Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Predicting immune-related thyroiditis using polygenic risk scores in patients with advanced melanoma.

Journal for immunotherapy of cancer·2026

Same author

Immune Checkpoint Inhibitor Esophagitis: Clinical and Endoscopic Presentation, Treatment Outcomes, and Complications.

Clinical and translational gastroenterology·2026

Same author

Same-Slide Spatial Multi-Omics Integration with IN-DEPTH Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment.

Cancer discovery·2026

Same author

Insights into inherited genetic variations and genetic ancestry of patients with high-risk melanoma.

Melanoma research·2026

Same author

Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancer.

Cancer cell·2026

Same author

On the Significance of Amino Acids in Insects.

Archives of insect biochemistry and physiology·2026

Same journal

Protocol for ultrasound-guided injection into the murine portal vein to initiate liver metastasis.

STAR protocols·2026

Same journal

Protocol for semi-automatic quantitative bioimaging analysis of synapse loss.

STAR protocols·2026

Same journal

Protocol for integrated ubiquitination analysis of in vitro E3 ligase-DUB regulation and in vivo ubiquitin chain linkage characterization.

STAR protocols·2026

Same journal

Protocol for constructing multi-ancestry polygenic models using S4-Multi.

STAR protocols·2026

Same journal

Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model.

STAR protocols·2026

Same journal

Overcoming the challenges of genome-editing essential genes.

STAR protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 3, 2025

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples.

Emily M Thrash¹, Katja Kleinsteuber¹, Emma S Hathaway¹

¹Center for Immuno-Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.

|October 28, 2020

Summary

This summary is machine-generated.

This study introduces a streamlined mass cytometry (MC) workflow using reference sample spike-in and cell barcoding to reduce batch effects. The method ensures reproducible data for up to 80 samples in high-throughput clinical settings.

More Related Videos

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Related Experiment Videos

Last Updated: Dec 3, 2025

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Area of Science:

Biomedical research
Clinical diagnostics
Immunology

Background:

Implementing mass cytometry (MC) in clinical settings requires robust protocols to minimize batch effects.
Reproducibility is crucial for reliable clinical data interpretation.

Purpose of the Study:

To present a streamlined, high-throughput mass cytometry workflow.
To reduce batch effects and improve data reproducibility in clinical MC applications.

Main Methods:

Developed a workflow combining reference sample spike-in and palladium-based mass-tag cell barcoding.
Applied the method for high-throughput staining of up to 80 samples per experiment.

Main Results:

The workflow generates reproducible data by decreasing experimental variables.
Successfully mitigated batch effects in a high-throughput setting.

Conclusions:

The presented streamlined MC workflow enhances data reproducibility for clinical implementation.
This method is valuable for reducing technical error and batch effects in large-scale sample analysis.