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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

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Related Experiment Video

Updated: Dec 3, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

The Long Non-Coding RNA NEAT1 Promotes Gastric Cancer Cell Proliferation and Invasion by Regulating miR-103a/

Da-Jun Yu¹, Chen-Xu Guo¹, Jun Qian¹

¹Department of Surgical Oncology, 74540The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.

Technology in Cancer Research & Treatment

|October 28, 2020

Summary

Long non-coding RNA NEAT1 promotes gastric cancer progression by enhancing proliferation and metastasis. Inhibiting NEAT1 or overexpressing miR-103a reduces cancer cell malignancy, suggesting NEAT1 as a potential biomarker for gastric cancer.

Keywords:

LncRNA NEAT1 STAMBPL1 gastric cancer invasion miR-103a proliferation

Related Experiment Videos

Last Updated: Dec 3, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

Molecular Biology
Oncology
Genetics

Background:

Gastric cancer (GC) is a prevalent malignancy with significant morbidity.
Long non-coding RNAs (LncRNAs) play crucial roles in cancer development.
The specific role of LncRNA NEAT1 in GC tumorigenesis requires investigation.

Purpose of the Study:

To investigate the effect of LncRNA NEAT1 on the proliferation and metastasis of gastric cancer.
To explore the regulatory relationship between NEAT1, miR-103a, and STAMBPL1 in GC.

Main Methods:

Examined NEAT1 expression in clinical GC samples and cell lines.
Utilized luciferase reporter assays to determine interactions between NEAT1, miR-103a, and STAMBPL1.
Assessed cell viability, invasion, and STAMBPL1 protein levels following NEAT1 knockdown and miR-103a overexpression.

Main Results:

LncRNA NEAT1 expression was significantly upregulated in GC cell lines.
NEAT1 was identified as a direct target of miR-103a.
NEAT1 knockdown and miR-103a overexpression suppressed GC cell proliferation and invasion, reducing STAMBPL1 levels.

Conclusions:

LncRNA NEAT1 acts as an oncogene in gastric cancer, promoting malignant behaviors.
NEAT1 is targeted by miR-103a and its oncogenic role may involve STAMBPL1.
NEAT1 shows potential as a biomarker for predicting gastric cancer progression.