Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

604
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
604
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

The Epidemiological and Toxicological Intersection of Air Pollution and Dementia.

Reviews of environmental contamination and toxicology·2026
Same author

Correcting curvature in micromirror-based spatial light modulators with a microlens array.

Optics express·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Perturbative Fourier ptychographic microscopy for fast quantitative phase imaging.

Optics express·2025
Same author

Space-time reconstruction for lensless imaging using implicit neural representations.

Optics express·2025

Related Experiment Video

Updated: Dec 3, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

17.0K

Fourier DiffuserScope: single-shot 3D Fourier light field microscopy with a diffuser.

Fanglin Linda Liu, Grace Kuo, Nick Antipa

    Optics Express
    |October 29, 2020
    PubMed
    Summary
    This summary is machine-generated.

    Fourier DiffuserScope overcomes resolution loss in light field microscopy using a diffuser for enhanced 3D imaging. This novel approach achieves high-resolution imaging across a large volume, outperforming traditional methods.

    More Related Videos

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.0K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.2K

    Related Experiment Videos

    Last Updated: Dec 3, 2025

    Determining 3D Flow Fields via Multi-camera Light Field Imaging
    14:25

    Determining 3D Flow Fields via Multi-camera Light Field Imaging

    Published on: March 6, 2013

    17.0K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.0K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.2K

    Area of Science:

    • Microscopy and Imaging Technologies
    • Optical Physics
    • Computational Imaging

    Background:

    • Light field microscopy (LFM) enables single-shot 3D imaging but suffers from reduced lateral resolution.
    • Conventional LFM configurations often compromise field-of-view (FOV) or introduce artifacts.
    • Optimizing LFM requires balancing resolution, FOV, and axial range.

    Purpose of the Study:

    • To develop a novel 3D imaging technique with improved resolution over a large volume.
    • To introduce Fourier DiffuserScope as an alternative to LFM with enhanced performance.
    • To establish theoretical guidelines for diffuser design in 3D microscopy.

    Main Methods:

    • Utilized a diffuser composed of randomly placed microlenses with varying focal lengths in the pupil plane.
    • Employed computational reconstruction by solving a sparsity-constrained inverse problem.
    • Developed a theoretical framework and design guidelines for diffuser parameters.

    Main Results:

    • Achieved < 3 µm lateral and 4 µm axial resolution over a 1000 × 1000 × 280 µm³ volume.
    • Demonstrated superior performance compared to microlens array (MLA) based LFM.
    • Showcased uniform resolution across a larger volume, both laterally and axially.

    Conclusions:

    • Fourier DiffuserScope offers significant advantages over LFM for high-resolution 3D imaging.
    • The developed theoretical framework aids in optimizing diffuser-based microscopy systems.
    • This technique provides a powerful tool for volumetric imaging with enhanced fidelity.