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LINC02738 Participates in the Development of Kidney Cancer Through the miR-20b/Sox4 Axis

Chao Han1, Bin Xu1, Lin Zhou1

  • 1Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People's Republic of China.

Oncotargets and Therapy
|October 29, 2020
PubMed
Abstract

Insights

High expression of LINC02783 in renal cell carcinoma (RCC) correlates with poor prognosis. This long non-coding RNA promotes RCC progression via the miR-20b/Sox-4 pathway, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play a role in tumorigenesis.
  • The Cancer Genome Atlas (TCGA) data indicate elevated LINC02783 expression in renal cell carcinoma (RCC).
  • LINC02783 is a potential therapeutic target in RCC.

Purpose of the Study:

  • To verify the high expression of LINC02783 in RCC.
  • To investigate the role of LINC02783 in RCC progression.
  • To explore the underlying molecular mechanism of LINC02783 in RCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for LINC02783 expression.
  • Cell viability (CCK-8) and invasion (Transwell) assays.
  • Western blot, dual-luciferase reporter (DLR) assay, RNA immunoprecipitation (RIP), and in vivo tumor formation experiments.

Main Results:

  • LINC02783 was highly expressed in RCC tissues, correlating with poorer patient prognosis.
  • Downregulation of LINC02783 inhibited RCC cell viability and invasion in vitro and in vivo.
  • LINC02783 acts as a molecular sponge for miR-20b, thereby enhancing Sox-4 expression.

Conclusions:

  • LINC02783 is upregulated in RCC and associated with poor prognosis.
  • LINC02783 promotes RCC occurrence and progression through the miR-20b/Sox-4 axis.
  • LINC02783 represents a promising therapeutic target for RCC treatment.
Keywords:
LINC02783Sox-4The Cancer Genome AtlasmiR-20bprognosisrenal cell carcinoma

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