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Melatonin supplementation improved cryopreserved Thai swamp buffalo semen
Wilasinee Inyawilert1,2, Janyaporn Rungruangsak3, Yu-Jing Liao4
1Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.
Reproduction in Domestic Animals = Zuchthygiene
|October 29, 2020
Summary
Adding melatonin to semen extender protects swamp buffalo sperm during cryopreservation. The optimal 1 mM concentration improved sperm viability and motility, while higher doses were detrimental.
Area of Science:
- Reproductive Biology
- Biochemistry
Background:
- Cryopreservation induces oxidative stress via reactive oxygen species (ROS), impairing sperm quality.
- Melatonin, a potent antioxidant, shows potential in mitigating cryodamage to spermatozoa.
Purpose of the Study:
- To investigate the efficacy of melatonin supplementation in semen extender for improving swamp buffalo sperm quality post-cryopreservation.
- To determine the optimal melatonin concentration for protecting buffalo sperm during cryopreservation.
Main Methods:
- Semen from six swamp buffalo bulls was cryopreserved using a tris-citrate egg yolk extender with varying melatonin concentrations (0–3.0 mM).
- Sperm viability and motility were assessed using a computer-assisted semen analyzer (CASA) at multiple time points post-cryopreservation (days 1, 7, 15, 30).
Main Results:
- Supplementation with 1.0 mM melatonin significantly enhanced sperm viability and motility compared to controls.
- Higher melatonin concentrations (3.0 mM) negatively impacted all assessed sperm parameters.
Conclusions:
- 1.0 mM melatonin in semen extender provides optimal protection for swamp buffalo sperm against cryopreservation-induced damage.
- Melatonin supplementation is a promising strategy to improve the fertility of cryopreserved swamp buffalo semen.
