Related Experiment Videos
Synthesis and secretion of wheat alpha-amylase in Saccharomyces cerevisiae
S J Rothstein1, K N Lahners, C M Lazarus
1Plant Breeding Institute, Trumpington, Cambridge, U.K.
Gene
|January 1, 1987
Abstract:
A wheat alpha-amylase cDNA clone has been fused to the phosphoglycerate kinase initiator methionine to enable synthesis in the yeast Saccharomyces cerevisiae of an alpha-amylase enzyme that is identical in size to the wild-type alpha-amylase. The alpha-amylase is synthesized with an N-terminal plant signal peptide which is recognized in the yeast host, leading to efficient processing and secretion into the medium. The secretion of alpha-amylase into the medium is quite efficient in rich medium, but barely detectable in a minimal medium.