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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Dec 3, 2025

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Optogenetics and Optical Tools in Automated Patch Clamping.

Kim Boddum¹, Peder Skafte-Pedersen¹, Jean-Francois Rolland²

¹Sophion Bioscience A/S, Ballerup, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|October 29, 2020

Summary

This summary is machine-generated.

Automated patch clamping (APC) combined with optogenetics enhances control over cellular activity. This integration enables robust data collection for novel therapeutic applications and drug safety screening.

Keywords:

APC Automated patch clamp Caged compounds ChR2 Channelrhodopsin Fast ligand gated channels HCN phosphorylation Optogenetics Qube Opto 384 Rubi-GABA Spatiotemporal kinetics bPAC cAMP

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Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Published on: February 11, 2015

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Last Updated: Dec 3, 2025

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Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Published on: February 11, 2015

Area of Science:

Electrophysiology
Optogenetics
Pharmacology

Background:

Automated patch clamping (APC) has been a cornerstone for high-throughput electrophysiological measurements for nearly two decades, particularly in preclinical drug safety screening.
Traditional APC involves suctioning cells onto planar surfaces for whole-cell configuration to measure ion channel activity.
Optogenetic tools offer precise spatiotemporal control for in vivo and in vitro experiments, aiding the study of cellular networks, transduction, and subcellular pathways.

Purpose of the Study:

To present a novel automated patch clamping (APC) optogenetics capability.
To demonstrate the utility of this integrated system for investigating light-activated ion channels and photoactivated ligands.
To explore new applications and therapeutic areas enabled by enhanced control over cell kinetics.

Main Methods:

Integration of optogenetic tools with an automated patch clamping system (Qube Opto 384).
Utilizing light-activated ion channels for precise cellular stimulation.
Employing photoactivated ligands to modulate cellular responses.

Main Results:

Successful demonstration of APC optogenetics capability on the Qube Opto 384 system.
Acquisition of robust electrophysiological data using light-activated ion channels and photoactivated ligands.
Validation of the combined approach for improved investigation and control of cell kinetics.

Conclusions:

The combination of APC and optogenetics significantly enhances the investigation and control of cellular kinetics.
This integrated system opens avenues for robust data collection in new therapeutic areas and advanced drug discovery.
The presented capability provides a powerful platform for studying complex cellular mechanisms with high spatiotemporal precision.