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Identification and Purification of Potential Bioactive Peptide of Moringa oleifera Seed Extracts
Sangeeta Chandrashekar1,2, Raman Vijayakumar2, Ramachandran Chelliah1
1Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, Korea.
Abstract:
The aim of the study was to investigate the antibacterial and anticoagulant activity of Moringa (Moringa oleifera) seed extracts and coagulant protein for their potential application in water treatment. Pathogenic microorganisms were obtained from Ramachandra Hospital, Chennai, India. Bacterial cell aggregation and growth kinetics studies were employed for six bacterial strains with different concentrations of seed extracts and coagulant protein. Moringa seed extract and coagulant protein showed cell aggregation against six bacterial strains, whereas seed extract alone showed growth inhibition of all six bacterial strains for up to 6 h, compared to that of control. Escherichia coli and Salmonella para typhi B did not develop resistance against coagulant protein. The results imply that Moringa oleifera is likely an efficient low-molecular bioactive peptide (with <7.5 kDa plant-based coagulant and antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography-mass spectrometry and HPLC, with the corresponding sequences from Napin-1A peptide posing different degrees of antibacterial activity against different pathogenic organisms.
Insights
Moringa oleifera seed extracts and coagulant protein demonstrate antibacterial and anticoagulant properties, effectively inhibiting pathogenic bacteria in water treatment applications. These findings highlight Moringa
Area of Science:
- Biotechnology
- Environmental Science
- Microbiology
Background:
- Water treatment often relies on chemical coagulants and antimicrobials.
- Exploring natural alternatives like Moringa oleifera offers sustainable solutions.
- Pathogenic microorganisms pose significant risks in untreated water sources.
Purpose of the Study:
- To evaluate the antibacterial and anticoagulant activity of Moringa oleifera seed extracts and coagulant protein.
- To assess their potential for application in water treatment processes.
- To identify the bioactive components responsible for these activities.
Main Methods:
- Bacterial cell aggregation and growth kinetics studies were performed on six pathogenic bacterial strains.
- Different concentrations of Moringa oleifera seed extracts and coagulant protein were tested.
- Liquid chromatography-mass spectrometry and HPLC were used to analyze peptide sequences.
Main Results:
- Moringa oleifera seed extract and coagulant protein induced cell aggregation in all tested bacterial strains.
- Moringa oleifera seed extract inhibited bacterial growth for up to 6 hours.
- Escherichia coli and Salmonella paratyphi B did not develop resistance to the coagulant protein.
Conclusions:
- Moringa oleifera contains efficient low-molecular-weight bioactive peptides (<7.5 kDa) with coagulant and antimicrobial properties.
- These plant-based peptides, particularly Napin-1A sequences, exhibit varying degrees of antibacterial activity.
- Moringa oleifera presents a promising natural agent for water treatment due to its antimicrobial and coagulant capabilities.
