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Published on: May 4, 2018
Genome Sequence of Clinical Strain Pseudomonas aeruginosa NRD619
Laura Sisk-Hackworth1, Andrew Sue1, Saima Aslam2
1Department of Biology, San Diego State University, San Diego, California, USA.
Abstract:
Here, we report the complete genome sequence of the multidrug-resistant (MDR) strain Pseudomonas aeruginosa NRD619, assembled via long- and short-read hybrid assembly. P. aeruginosa is a Gram-negative bacterial pathogen that is a significant public health burden. NRD619 was isolated from a left ventricular assist device (LVAD) draining sinus tract.
Insights
We sequenced the genome of a multidrug-resistant Pseudomonas aeruginosa strain, NRD619, found in a medical device infection. This provides a key resource for understanding and combating this dangerous bacterial pathogen.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Pseudomonas aeruginosa is a Gram-negative bacterial pathogen causing significant public health issues.
- Multidrug-resistant (MDR) strains pose a growing threat in clinical settings.
Purpose of the Study:
- To report the complete genome sequence of the MDR P. aeruginosa strain NRD619.
- To provide a genomic resource for studying MDR P. aeruginosa.
Main Methods:
- Hybrid genome assembly using both long-read and short-read sequencing technologies.
- Isolation of P. aeruginosa NRD619 from a left ventricular assist device (LVAD) draining sinus tract.
Main Results:
- The complete genome sequence of P. aeruginosa NRD619 was successfully assembled.
- The genome provides insights into the genetic basis of multidrug resistance in this clinical isolate.
Conclusions:
- The availability of the NRD619 genome sequence facilitates further research into P. aeruginosa pathogenesis and resistance mechanisms.
- This genomic data aids in the development of strategies to combat MDR bacterial infections.
