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Quantification of Cooking Method Effect on COP Content in Meat Types Using Triple Quadrupole GC-MS/MS.
Shazamawati Zam Hashari1, Alina Abdul Rahim1, Goh Yong Meng2,3
1Food Biotechnology Program, Faculty of Science and Technology, University Sains Islam Malaysia, Bandar Baru Nilai 71800, Malaysia.
Frying processed foods like beef, sausages, and fish produces more cholesterol oxidation products (COPs) than boiling. Choosing boiling and leaner meats can reduce harmful COPs in your diet.
Area of Science:
- Food Science
- Analytical Chemistry
- Public Health
Background:
- Dietary cholesterol is vital, but its oxidation products (COPs) pose health risks.
- Understanding how cooking affects COP formation is crucial for food safety.
Purpose of the Study:
- To investigate the impact of cooking methods (boiling vs. frying) on COP levels in processed foods.
- To quantify specific COPs in minced beef, chicken sausages, and fish fillets.
Main Methods:
- Food samples were cooked using boiling and frying methods.
- Cholesterol oxidation products (COPs) were extracted via saponification.
- Six common COPs were quantified using gas chromatography-mass spectrometry (GC-MS/MS).
Main Results:
- Minced meat had the highest COP levels, particularly 7-keto and triol.
- Frying significantly increased triol and 7-keto levels compared to boiling across all samples.
- Fish fillets produced the fewest COPs due to lower cholesterol and higher unsaturated fatty acids.
Conclusions:
- Cooking method significantly influences COP formation, with frying being more detrimental.
- Boiling is a preferable cooking method to minimize COP generation.
- Selecting appropriate food types, like fish, can also reduce dietary COP exposure.
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