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Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Hyperspectral infrared microscopy with visible light.

Anna V Paterova1, Sivakumar M Maniam2,3, Hongzhi Yang1

  • 1Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), Singapore 138634, Singapore.

Science Advances
|October 31, 2020
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Summary
This summary is machine-generated.

This study presents a novel infrared hyperspectral microscopy method using visible light components. It enables efficient chemical mapping with high spatial resolution, overcoming limitations of current infrared techniques.

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Area of Science:

  • Spectroscopy
  • Microscopy
  • Optics

Background:

  • Hyperspectral microscopy offers detailed spectral information with high spatial resolution.
  • Infrared (IR) hyperspectral microscopy is powerful for chemical fingerprinting but faces challenges in efficiency and cost.
  • Existing IR light sources and detectors limit widespread adoption of IR hyperspectral imaging.

Purpose of the Study:

  • To develop a new, cost-effective approach for infrared (IR) hyperspectral microscopy.
  • To demonstrate chemical mapping using readily available visible light components.
  • To overcome the limitations of current IR hyperspectral imaging technologies.

Main Methods:

  • Utilizing nonlinear interference of correlated photons generated via parametric down-conversion.
  • Adapting off-the-shelf visible light components for IR spectral mapping.
  • Implementing a proof-of-concept chemical mapping of a patterned sample.

Main Results:

  • Successful chemical mapping of a patterned sample with distinct IR spectroscopic fingerprints.
  • Demonstration of a wide field of view and fast readout capabilities.
  • Negligible heat delivery to the sample, preserving sample integrity.

Conclusions:

  • The novel method provides a viable alternative for IR hyperspectral microscopy.
  • This technique offers advantages in cost, efficiency, and sample preservation.
  • The approach holds promise for advanced material and biological studies.