Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Productivity starts with people.

BMJ leader·2026

Same author

Label-Free Detection of Cellular Aβ Accumulation and Mitochondrial Dysfunction in AD Cell Models via Raman Microscopy.

Analytical chemistry·2026

Same author

Spatially multiplexed random-access Raman probe for in vivo tissue measurements.

Biomedical optics express·2026

Same author

Electrically switchable continuous phase liquid crystal Fresnel zone plate.

Light, science & applications·2026

Same author

Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection.

Cell reports·2026

Same author

A likelihood-based Bayesian inference framework for the calibration of and selection between stochastic velocity-jump models.

Journal of the Royal Society, Interface·2026

Search research articles

Related Experiment Video

Updated: Dec 3, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope.

Syed Asad Hussain¹, Toshiki Kubo², Nicholas Hall³

¹Department of Engineering Science, University of Oxford, Oxford, UK.

Journal of Microscopy

|October 31, 2020

Summary

Adaptive optics successfully corrects specimen-induced aberrations in spinning disk microscopy. This wavefront-sensorless approach enhances imaging quality, improving signal, contrast, and resolution for clearer microscopic views.

Keywords:

Adaptive Optics Confocal Microscope Deformable Mirror Laser-Free Spinning Disk Microscope Wavefront-sensorless

More Related Videos

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Related Experiment Videos

Last Updated: Dec 3, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Area of Science:

Microscopy and Imaging Technologies
Optical Physics
Biomedical Engineering

Background:

Specimen-induced aberrations compromise image quality in high-resolution microscopy.
Adaptive optics (AO) is a technique used to correct optical aberrations.
Spinning disk microscopy offers optical sectioning but is susceptible to aberrations.

Purpose of the Study:

To implement wavefront-sensorless adaptive optics (WS-AO) for the first time in a laser-free, aperture correlation, spinning disk microscope.
To evaluate the effectiveness of WS-AO in correcting specimen-induced aberrations in this specific microscopy setup.
To demonstrate improvements in imaging performance metrics such as signal level, contrast, and resolution.

Main Methods:

Implementation of a wavefront-sensorless adaptive optics system.
Integration with a laser-free, aperture correlation, spinning disk microscope.
Characterization of imaging performance before and after aberration correction.

Main Results:

Successful implementation of WS-AO in a spinning disk microscope.
Demonstrated correction of specimen-induced aberrations.
Significant improvements observed in signal level, image contrast, and resolution.

Conclusions:

Wavefront-sensorless adaptive optics is a viable method for enhancing spinning disk microscopy.
This technique effectively overcomes limitations imposed by specimen-induced aberrations.
The implementation leads to superior optical sectioning and higher quality imaging in microscopy.