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Updated: Dec 3, 2025
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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
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Media Selection in Ion Exchange Chromatography in a Single Microplate
Charlotte Cabanne1, Xavier Santarelli2
1Bordeaux INP, CBMN, UMR 5248, Pessac, France. charlotte.cabanne@bordeaux-inp.fr.
Methods in Molecular Biology (Clifton, N.J.)
|October 31, 2020
Summary
High-throughput process development in chromatography is advancing. A new method using 96-well filter microplates overcomes limitations in selecting ion exchange chromatographic media.
Area of Science:
- Biotechnology
- Chemical Engineering
- Analytical Chemistry
Background:
- High-throughput process development is increasingly adopted in chromatography.
- Current tools from manufacturers present limitations for efficient media selection.
Purpose of the Study:
- To describe a novel method for selecting ion exchange chromatographic media.
- To address the limitations of existing tools in high-throughput chromatography.
Main Methods:
- Utilized a 96-well filter microplate format.
- Developed a streamlined process for ion exchange media screening.
Main Results:
- Successfully demonstrated a method for rapid selection of chromatographic media.
- The 96-well microplate approach facilitates efficient screening.
Conclusions:
- The described method offers a viable solution for high-throughput ion exchange media selection.
- This approach enhances process development efficiency in chromatography.
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