Related Experiment Video
Updated: Dec 2, 2025
08:15
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Published on: May 22, 2017
8.3K
Fluorescent Probes for Super-Resolution Microscopy of Lysosomes
Aditya Yadav1,2, Chethana Rao1,2, Chayan K Nandi1,3,2
1School of Basic Sciences, Indian Institute of Technology Mandi, Mandi 175075, H.P., India.
ACS Omega
|November 2, 2020
Summary
Understanding lysosome structure is crucial for neurodegenerative disease research. Super-resolution microscopy and advanced fluorescent probes offer new ways to visualize these vital organelles at the subnanometer level.
Area of Science:
- Cell Biology
- Neuroscience
- Microscopy
Background:
- Lysosomes are essential cytoplasmic organelles whose dysfunction is linked to neurodegenerative diseases.
- Subnanometer structural understanding of lysosomes is critical for disease research.
- Super-resolution imaging techniques allow visualization of suborganelle dynamics in living cells.
Purpose of the Study:
- To review super-resolution microscopy techniques for lysosome imaging.
- To discuss fluorescent probes for specific lysosome staining and resolution.
- To highlight advancements in visualizing lysosome structure and dynamics.
Main Methods:
- Review of super-resolution microscopy techniques (e.g., STED, STORM, PALM).
- Analysis of fluorescent probe properties (brightness, photostability, specificity).
- Application of these techniques to visualize lysosome ultrastructure.
Main Results:
- Super-resolution microscopy breaks the diffraction limit for subnanometer imaging.
- Development of brighter, photostable probes enhances lysosome visualization.
- These methods reveal dynamic processes within lysosomes at unprecedented resolution.
Conclusions:
- Super-resolution imaging is vital for understanding lysosome structure and function.
- Appropriate fluorescent probes are key to achieving high-resolution lysosome imaging.
- Advancements in imaging offer new insights into lysosome-related diseases.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
01:19Protein Dynamics in Living Cells
2.5K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.5K