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Related Experiment Video

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Bioprinting a Multifunctional Bioink to Engineer Clickable 3D Cellular Niches with Tunable Matrix Microenvironmental

Rúben F Pereira1,2,3, Bianca N Lourenço1,2, Paulo J Bártolo4,5

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, 4200-135, Portugal.

Advanced Healthcare Materials
|November 2, 2020
PubMed
Summary

Researchers developed a novel bioink for 3D bioprinting, enabling independent control over biochemical and biophysical cues. This allows for precise tuning of the cell environment to study cell behavior in engineered tissues.

Keywords:
biofabricationbioinksclick chemistryextracellular matrixhydrogels

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Cellular responses in 3D environments are crucial but difficult to study in bioprinted constructs.
  • Existing extracellular matrix (ECM)-mimetic bioinks lack fully controllable properties.
  • Understanding how microenvironmental cues influence cell fate is essential for tissue engineering.

Purpose of the Study:

  • To design a multifunctional bioink for extrusion bioprinting with independent control over biochemical and biophysical cues.
  • To create bioinspired 3D cellular niches with tunable properties.
  • To investigate the impact of matrix stiffness on cell proliferation and ECM deposition.

Main Methods:

  • Utilized thiol-norbornene photoclick chemistry for bioorthogonal reactions.
  • Controlled bioink rheology via ionic gelation with divalent ions (calcium and barium).
  • Tailored mechanical and biochemical properties through post-printing thiol-ene reactions.

Main Results:

  • Developed a bioink allowing independent tuning of biochemical and biophysical properties.
  • Created cell-adhesive and protease-degradable hydrogels.
  • Demonstrated matrix stiffness-dependent modulation of cell proliferation and ECM deposition over 14 days, irrespective of cell spreading.

Conclusions:

  • The developed bioink serves as a versatile platform for bioprinting functional tissue constructs.
  • Enables precise control over cellular behavior by modulating the microenvironment.
  • Facilitates probing the effects of matrix cues on cell responses in 3D cultures.