Modulation of Proinflammatory Bacteria- and Lipid-Coupled Intracellular Signaling Pathways in a Transwell Triple
Darab Ghadimi1, Annegret Nielsen1, Mohamed Farghaly Yoness Hassan1
1Department of Microbiology and Biotechnology, Max Rubner-Institute, Hermann-Weigmann-Str 1, D-24103 Kiel, Germany.
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry
|November 2, 2020
Summary
Commensal Bifidobacterium animalis R101-8 reduces inflammation markers by metabolizing fatty acids and inhibiting key signaling pathways. This probiotic enhances cellular tolerance to excess lipids, aiding metabolic homeostasis.
Area of Science:
- Microbiology
- Immunology
- Metabolic Science
Background:
- High-fat diets alter gut microbiota, increasing gut permeability, endotoxemia, and low-grade inflammation.
- Commensal bifidobacteria's role in modulating metaflammation biomarkers and inflammatory pathways requires further investigation.
Purpose of the Study:
- To investigate how Bifidobacterium animalis R101-8 influences metaflammation biomarkers (chemerin, MCP-1, PEDF).
- To assess the impact of B. animalis R101-8 on pro-inflammatory signaling pathways in an inflamed adipose tissue model.
Main Methods:
- A triple co-culture model of intestinal, immune, and adipose cells was used.
- Cells were exposed to B. animalis R101-8 with lipopolysaccharide (LPS) or palmitic acid.
- Gene and protein expression of inflammatory markers and signaling pathways were analyzed via qRT-PCR, ELISA, and Western blotting.
Main Results:
- B. animalis R101-8 inhibited LPS- and palmitic acid-induced inflammatory cytokines (IL-1β, IL-6, TNF-α).
- It reduced biomarkers chemerin, MCP-1, PEDF, and cellular triglycerides.
- B. animalis R101-8 blocked NF-kB and AP-1 activation pathways and downregulated key lipid metabolism and inflammatory genes.
Conclusions:
- B. animalis R101-8 mitigates metaflammation through fatty acid metabolism and inhibition of NF-kB and AP-1 signaling.
- This probiotic enhances cellular tolerance to pro-inflammatory lipids.
- B. animalis R101-8 plays a role in the homeostatic regulation of metabolic processes.
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